Analytical Data
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Gene name
USP5
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简介
USP5 protein selectively cleaves branched multiubiquitin polymers, favoring 'Lys-48'-linked polyubiquitin disassembly. It exhibits lower affinity for linear and 'Lys-63'-linked polyubiquitin. USP5 knockdown leads to p53/TP53 accumulation, enhancing p53/TP53 transcriptional activity, as unanchored polyubiquitin competes with ubiquitinated p53/TP53 for proteasomal recognition, unlike MDM2. USP5 Protein, Human (His) is the recombinant human-derived USP5 protein, expressed by E. coli, with N-His labeled tag.
- Application
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Alternative Names
Ubiquitin carboxyl-terminal hydrolase 5; Deubiquitinating enzyme 5; Isopeptidase T; Ubiquitin thioesterase 5; Ubiquitin-specific-processing protease 5; USP5; ISOT
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P45974-1
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Expression Region
A2-S858
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AA Sequence
AELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGERVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKGSLGFYGNEDEDSFCSPHFSSPTSPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVAS
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Protein Length
Full Length of Isoform-1 Mature Protein
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
USP5 (Ubiquitin-specific protease 5) is a crucial enzyme within the ubiquitin-proteasome system, playing a significant role in regulating protein degradation and cellular homeostasis. It specifically deubiquitinates substrates, reversing the action of ubiquitin ligases, thereby influencing various cellular processes such as signal transduction, gene expression, and cell cycle progression. Abnormal USP5 activity has been linked to several diseases, including cancer, neurodegenerative disorders, and infections, highlighting its potential as a therapeutic target. Recent studies have focused on the detailed characterization of USP5 structure and function, aiming to elucidate its substrates and regulatory mechanisms. The development of USP5 recombinant proteins has enabled researchers to investigate its enzymatic activity and interactions with other proteins, providing insights into its biological roles and potential as a drug target. Enhanced understanding of USP5's molecular mechanisms may pave the way for novel treatments that manipulate proteasomal pathways, thus contributing to disease management and therapy advancement. Consequently, the ongoing research on USP5 recombinant proteins is vital for uncovering new strategies in targeted therapy and improving our understanding of cellular regulation.
