Analytical Data
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基因名
EIF3G
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简介
The EIF3G protein is an RNA-binding component of the eIF-3 complex that initiates protein synthesis by promoting the recruitment of factors to form the 43S PIC. EIF3G is critical in both mRNA recruitment and AUG recognition scanning. EIF3G Protein, Human (His-SUMO) is the recombinant human-derived EIF3G protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.
- Application
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种属
Human
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表达系统
E. coli
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标签
N-SUMO;N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75821
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表达区间
P2-N320
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氨基酸序列
PTGDFDSKPSWADQVEEEGEDDKCVTSELLKGIPLATGDTSPEPELLPGAPLPPPKEVINGNIKTVTEYKIDEDGKKFKIVRTFRIETRKASKAVARRKNWKKFGNSEFDPPGPNVATTTVSDDVSMTFITSKEDLNCQEEEDPMNKLKGQKIVSCRICKGDHWTTRCPYKDTLGPMQKELAEQLGLSTGEKEKLPGELEPVQATQNKTGKYVPPSLRDGASRRGESMQPNRRADDNATIRVTNLSEDTRETDLQELFRPFGSISRIYLAKDKTTGQSKGFAFISFHRREDAARAIAGVSGFGYDHLILNVEWAKPSTN
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蛋白长度
Full Length of Mature Protein
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EIF3G, or Eukaryotic Translation Initiation Factor 3 Subunit G, is a critical component of the eukaryotic translation initiation machinery. It plays a significant role in the recruitment of mRNA and the assembly of the ribosomal initiation complex, making it essential for the overall protein synthesis process. Research into EIF3G has garnered attention due to its involvement in various cellular processes, including cell proliferation, differentiation, and survival. Importantly, EIF3G has been implicated in several diseases, notably cancer, where its overexpression can contribute to tumor progression and metastasis. Given its role in translation and its potential as a therapeutic target, scientists have focused on characterizing recombinant EIF3G to better understand its structure and function. The study of its recombinant forms allows for detailed investigations into the mechanisms by which EIF3G interacts with other initiation factors and mRNA, facilitating the elucidation of its precise role in translation regulation. Furthermore, recombinant EIF3G can be utilized in drug discovery efforts aimed at modulating its activity, providing a valuable avenue for developing innovative cancer therapies. Understanding the biochemical properties and interactions of EIF3G through recombinant protein studies is crucial to advancing our knowledge of translation initiation and its implications in health and disease.
