Analytical Data
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Gene name
AGO2/Argonaute-2
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简介
The AGO2/Argonaute-2 protein is essential for RNA-mediated gene silencing via the RNA-induced silencing complex (RISC). "Minimal RISC" involves AGO2 binding to a guide RNA, such as microRNA (miRNA) or short interfering RNA (siRNA), which guides RISC for silencing with complementary mRNA. AGO2/Argonaute-2 Protein, Mouse (sf9, His) is the recombinant mouse-derived AGO2/Argonaute-2 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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Alternative Names
Protein argonaute-2; Argonaute2; mAgo2; eIF-2C 2; Eif2c2
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Species
Mouse
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Source
Baculovirus
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8CJG0
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Expression Region
M1-A860
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Protein Length
Full Length
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Molecular Weight
105 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AGO2, or Argonaute-2, is a key protein involved in the RNA silencing pathways, particularly in the microRNA (miRNA) and small interfering RNA (siRNA) pathways. As a crucial component of the RNA-induced silencing complex (RISC), AGO2 plays a vital role in regulating gene expression by binding to small RNA molecules and guiding them to complementary mRNA targets, leading to mRNA degradation or translational repression. The significance of AGO2 in various biological processes, including development, differentiation, and immune responses, has made it a focal point in understanding post-transcriptional regulation. Research into AGO2 has revealed its essential role in tumorigenesis, as alterations in miRNA expression can significantly impact cancer progression. Therefore, the study of AGO2 and its interactions with small RNAs is critical for elucidating the mechanisms of gene regulation and for developing potential therapeutic strategies to target diseases associated with dysregulated RNA silencing pathways. With advances in biochemical techniques, the reconstitution of AGO2 and its complexes has become feasible, enabling detailed studies of its function and regulation in cellular contexts. This research is not only important for basic biology but also has implications for the development of RNA-based therapeutics in treating various diseases, including cancer and viral infections.
