Analytical Data
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基因名
AGO2/Argonaute-2
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简介
The AGO2/Argonaute-2 protein is essential for RNA-mediated gene silencing via the RNA-induced silencing complex (RISC). "Minimal RISC" involves AGO2 binding to a guide RNA, such as microRNA (miRNA) or short interfering RNA (siRNA), which guides RISC for silencing with complementary mRNA. AGO2/Argonaute-2 Protein, Mouse (sf9, His) is the recombinant mouse-derived AGO2/Argonaute-2 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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别名
Protein argonaute-2; Argonaute2; mAgo2; eIF-2C 2; Eif2c2
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种属
Mouse
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表达系统
Baculovirus
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8CJG0
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表达区间
M1-A860
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蛋白长度
Full Length
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分子量
105 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AGO2, or Argonaute-2, is a key protein involved in the RNA silencing pathways, particularly in the microRNA (miRNA) and small interfering RNA (siRNA) pathways. As a crucial component of the RNA-induced silencing complex (RISC), AGO2 plays a vital role in regulating gene expression by binding to small RNA molecules and guiding them to complementary mRNA targets, leading to mRNA degradation or translational repression. The significance of AGO2 in various biological processes, including development, differentiation, and immune responses, has made it a focal point in understanding post-transcriptional regulation. Research into AGO2 has revealed its essential role in tumorigenesis, as alterations in miRNA expression can significantly impact cancer progression. Therefore, the study of AGO2 and its interactions with small RNAs is critical for elucidating the mechanisms of gene regulation and for developing potential therapeutic strategies to target diseases associated with dysregulated RNA silencing pathways. With advances in biochemical techniques, the reconstitution of AGO2 and its complexes has become feasible, enabling detailed studies of its function and regulation in cellular contexts. This research is not only important for basic biology but also has implications for the development of RNA-based therapeutics in treating various diseases, including cancer and viral infections.
