Analytical Data
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基因名
RecR
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简介
RecR protein potentially plays a vital role in DNA repair, especially in RecBC-independent recombinational processes. It collaborates with RecF and RecO, suggesting its involvement in cooperative interactions within the cellular machinery dedicated to DNA repair. Further investigation is needed to elucidate RecR's precise functions and molecular interactions in maintaining genomic integrity through DNA repair. RecR Protein, E.coli (His-SUMO) is the recombinant E. coli-derived RecR protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.
- Application
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别名
recR; b0472; JW0461; Recombination protein RecR
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种属
E.coli
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表达系统
E. coli
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标签
N-SUMO;N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A7H6
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表达区间
M1-F201
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氨基酸序列
MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLAQALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFSGRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAELCAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF
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蛋白长度
Full Length
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分子量
38.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RecR is a crucial protein involved in homologous recombination, a vital process for DNA repair and genetic diversity in prokaryotes. It plays a significant role in ensuring the accurate repair of DNA double-strand breaks, which can arise from various sources such as environmental stresses, replication errors, or radiation damage. The RecR protein functions as a single-stranded DNA (ssDNA) binding protein, facilitating the assembly of the recombinase complex that includes other key proteins, such as RecA. Understanding the biochemical properties and mechanisms of RecR is essential for elucidating the recombination process at a molecular level. Furthermore, investigations into the structure and function of RecR can reveal insights into the broader implications of genome stability, potential evolutionary adaptations, and the development of novel antimicrobial strategies. Given its importance, RecR has garnered attention in microbiological and genetic research, making it a relevant subject for developing plasmid-based gene transfer techniques and synthetic biology applications. The ongoing studies of RecR and its interactions with other recombination proteins continue to enhance our understanding of genetic repair pathways and their evolutionary significance across different organisms.
