Analytical Data
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基因名
STC1/Stanniocalcin-1
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简介
STC1/Stanniocalcin-1 Proteinas, a homodimer, crucially stimulates renal phosphate reabsorption, safeguarding against hypercalcemia. Disulfide linkages contribute to its structural integrity. Its role in renal processes underscores significance in regulating phosphate levels, emphasizing a key role in maintaining mineral homeostasis. STC1/Stanniocalcin-1 Protein, Human (HEK293, His) is the recombinant human-derived STC1/Stanniocalcin-1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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生物活性
1.Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is 1.019 ng/mL, corresponding to a specific activity is 9.81×105 U/mg. 2.Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 30.85 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a. corresponding to a specific activity is 3.241×104 units/mg. Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is 1.019 ng/mL, corresponding to a specific activity is 9.81×105 U/mg.
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别名
Stanniocalcin 1; stanniocalcin-1; STC1; STC-1; STCSTC-1
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P52823-1
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表达区间
T18-A247
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氨基酸序列
THEAEQNDSVSPRKSRVAAQNSAEVVRCLNSALQVGCGAFACLENSTCDTDGMYDICKSFLYSAAKFDTQGKAFVKESLKCIANGVTSKVFLAIRRCSTFQRMIAEVQEECYSKLNVCSIAKRNPEAITEVVQLPNHFSNRYYNRLVRSLLECDEDTVSTIRDSLMEKIGPNMASLFHILQTDHCAQTHPRADFNRRRTNEPQKLKVLLRNLRGEEDSPSHIKRTSHESA
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蛋白长度
Full Length of Isoform-1 Mature Protein
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分子量
30-40 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Stanniocalcin-1 (STC1) is a glycoprotein originally identified in fish, playing a crucial role in calcium and phosphate homeostasis. In lower vertebrates, STC1 is known to regulate mineral metabolism, particularly in response to environmental changes. Recent studies have shown that STC1 also has significant implications in human physiology, influencing various cellular processes such as proliferation, differentiation, and apoptosis. Its dysregulation has been linked to several diseases, including cancer and bone disorders, making it a target of interest in biomedical research. The recombinant version of STC1, produced through recombinant DNA technology, allows for extensive in vitro studies, enabling researchers to investigate its structure-function relationships and therapeutic potential. Furthermore, understanding STC1's signaling pathways could provide insights into its role in cellular homeostasis and its mechanisms in disease progression. As such, the study of STC1 and its recombinant forms is essential for advancing knowledge in endocrinology, oncology, and tissue regeneration, establishing a foundation for developing novel therapeutic strategies.
