Analytical Data
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基因名
Alpha-crystallin A chain/CRYAA
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简介
Alpha-crystallin A chain/CRYAA Protein, Human (His) is a recombinant Alpha-crystallin A chain protein with a His tag. Alpha-crystallin A chain/CRYAA is a small heat shock protein and molecular chaperone that prevents nonspecific aggregation of denaturing proteins. Several point mutations in the alphaA-crystallin gene cause congenital human cataracts by unknown mechanisms[1].
- Application
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生物活性
Measured by its binding ability in a functional ELISA. When Recombinant CRYAA Human is used at 2 μg/mL can bind anti-CRYAA antibody. The ED50 for this effect is 1.405 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant CRYAA Human is used at 2 μg/mL can bind anti-CRYAA antibody. The ED50 for this effect is 1.405 μg/mL.
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别名
rHuAlpha-crystallin A chain/CRYAA, His; Alpha-Crystallin A Chain; Heat Shock Protein Beta-4; HspB4; Alpha-Crystallin A Chain; Short Form; CRYAA; CRYA1; HSPB4
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种属
Human
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表达系统
E. coli
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P02489
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表达区间
M1-S173
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蛋白长度
Full Length
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分子量
20-23 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Alpha-crystallin A chain (CRYAA) is a crucial component of the eye lens, playing a vital role in maintaining lens transparency and preventing protein aggregation, which is essential for proper vision. CRYAA belongs to the small heat shock protein family, exhibiting chaperone-like properties that help protect cellular proteins from stress-induced damage. Research on the recombinant form of CRYAA has gained significance due to its potential therapeutic applications in ocular diseases, particularly cataract formation, which remains a leading cause of blindness globally. The ability to produce recombinant CRYAA allows for detailed structural and functional studies, enabling scientists to understand its protective mechanisms at a molecular level. Furthermore, studies have indicated that CRYAA might have implications beyond the lens, influencing cellular stress responses and playing a role in various pathological conditions, including neurodegenerative diseases. The exploration of CRYAA's functions through recombinant protein allows for innovative approaches to drug development and the design of novel therapies aimed at enhancing visual health and overall protein homeostasis. This research highlights the importance of CRYAA as a multi-functional protein, bridging gaps between basic and clinical science, ultimately paving the way for advancements in treatments for eye disorders and age-related conditions, thus improving patient outcomes.
