Analytical Data
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Gene name
Alpha-crystallin A chain/CRYAA
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简介
Alpha-crystallin A chain/CRYAA Protein, Human (His) is a recombinant Alpha-crystallin A chain protein with a His tag. Alpha-crystallin A chain/CRYAA is a small heat shock protein and molecular chaperone that prevents nonspecific aggregation of denaturing proteins. Several point mutations in the alphaA-crystallin gene cause congenital human cataracts by unknown mechanisms[1].
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. When Recombinant CRYAA Human is used at 2 μg/mL can bind anti-CRYAA antibody. The ED50 for this effect is 1.405 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant CRYAA Human is used at 2 μg/mL can bind anti-CRYAA antibody. The ED50 for this effect is 1.405 μg/mL.
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Alternative Names
rHuAlpha-crystallin A chain/CRYAA, His; Alpha-Crystallin A Chain; Heat Shock Protein Beta-4; HspB4; Alpha-Crystallin A Chain; Short Form; CRYAA; CRYA1; HSPB4
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Species
Human
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Source
E. coli
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P02489
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Expression Region
M1-S173
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Protein Length
Full Length
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Molecular Weight
20-23 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Alpha-crystallin A chain (CRYAA) is a crucial component of the eye lens, playing a vital role in maintaining lens transparency and preventing protein aggregation, which is essential for proper vision. CRYAA belongs to the small heat shock protein family, exhibiting chaperone-like properties that help protect cellular proteins from stress-induced damage. Research on the recombinant form of CRYAA has gained significance due to its potential therapeutic applications in ocular diseases, particularly cataract formation, which remains a leading cause of blindness globally. The ability to produce recombinant CRYAA allows for detailed structural and functional studies, enabling scientists to understand its protective mechanisms at a molecular level. Furthermore, studies have indicated that CRYAA might have implications beyond the lens, influencing cellular stress responses and playing a role in various pathological conditions, including neurodegenerative diseases. The exploration of CRYAA's functions through recombinant protein allows for innovative approaches to drug development and the design of novel therapies aimed at enhancing visual health and overall protein homeostasis. This research highlights the importance of CRYAA as a multi-functional protein, bridging gaps between basic and clinical science, ultimately paving the way for advancements in treatments for eye disorders and age-related conditions, thus improving patient outcomes.
