Analytical Data
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Gene name
NIT2
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简介
The NIT2 protein has the extraordinary ability to catalyze the conversion of 3-indole-acetonitrile into 3-indole-acetic acid, an important plant hormone involved in many physiological processes. This transition is significant because it represents a key step in the biosynthesis of indole-3-acetic acid, a key regulator of plant growth and development. NIT2 Protein, Arabidopsis thaliana is the recombinant NIT2 protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
NIT2; Nitrilase 2
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Species
Others
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P32962
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Expression Region
M1-K339
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NIT2, a protein found in certain organisms, plays a crucial role in various biological processes, including nitrogen metabolism and stress response. As a member of the nitrogen regulatory protein family, NIT2 is particularly significant in fungi and plants, where it contributes to the regulation of nitrogen utilization under varying environmental conditions. Research on NIT2 has gained momentum due to the increasing importance of nitrogen management in agriculture, given its impact on crop yield and sustainability. Understanding the structure and function of NIT2 can provide insights into how organisms adapt to nutrient limitations and can inform biotechnological approaches aimed at enhancing nitrogen efficiency in crops. Recent advances in recombinant protein technology have enabled the production and characterization of NIT2, allowing scientists to investigate its specific molecular mechanisms and interactions within cellular pathways. This research not only furthers our comprehension of nitrogen regulation but also holds potential for developing innovative strategies to optimize nitrogen use in agricultural practices, thereby addressing global food security and environmental challenges associated with nitrogen overuse.
