Analytical Data
-
Gene name
Neurturin
-
简介
Neurturin protein, vital in neurobiology, is key to supporting sympathetic neuron survival and implicated in regulating the central nervous system (CNS). It influences neural growth, stability, and may modulate non-neuronal cell populations. Neurturin, a homodimer linked by disulfide bonds, maintains structural integrity for diverse cellular functions beyond neuronal survival. Neurturin Protein, Human is the recombinant human-derived Neurturin protein, expressed by E. coli , with tag free.
- Application
-
Biological Activity
Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is 31.8 ng/mL in the presence of 100 ng/mL Human GFR alpha-2, corresponding to a specific activity is 3.145×104 units/mg. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is 31.8 ng/mL in the presence of 100ng/mL Human GFR alpha-2, corresponding to a specific activity is 3.145×104 units/mg.
-
Alternative Names
Neurturin; NRTN
-
Species
Human
-
Source
E. coli
-
Tag
Tag Free
-
Purity
Greater than 95% as determined by reducing SDS-PAGE.
-
Uniprot
Q99748
-
Expression Region
A96-V197
-
Protein Length
Full Length of Mature Protein
-
Molecular Weight
15 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Neurturin is a member of the glial cell line-derived neurotrophic factor (GDNF) family, which plays a crucial role in the survival and maintenance of various neuronal populations, particularly those in the peripheral nervous system. Research into Neurturin emerged from the need to understand its neuroprotective properties and potential therapeutic applications for neurodegenerative diseases. Unlike other neurotrophic factors, Neurturin exhibits selective actions that promote the survival of specific neuronal subtypes, making it a candidate for targeted therapies in conditions like Parkinson's disease and amyotrophic lateral sclerosis (ALS). The study of Neurturin has focused on its mechanism of action, receptor binding, and downstream signaling pathways, revealing its ability to activate the Ret receptor tyrosine kinase and modulate pathways involved in neuronal survival and differentiation. Moreover, preclinical studies have shown promising results in animal models, where Neurturin administration helped restore motor functions and neuronal integrity. As research progresses, understanding Neurturin’s role in neural development and its potential to repair or regenerate damaged neurons continues to be a significant area of interest, paving the way for innovative treatment strategies in neurodegenerative conditions.
