Analytical Data
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Gene name
CDK4-CCND1
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简介
CDK4 protein, acting as a Ser/Thr kinase in the cyclin D-CDK4 complex, critically regulates the G(1)/S transition by phosphorylating and inhibiting RB family members. This activity, specifically hypophosphorylation of RB1 during early G(1) phase, releases E2F and promotes transcription of genes that drive G(1) progression. CDK4-CCND1 Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK4-CCND1, expressed by Sf9 insect cells, with N-His, N-GST, N-Flag labeled tag.
- Application
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Alternative Names
CDK4; cyclin-dependent kinase 4; PSK J3; cell division protein kinase 4; CMM3; PSK-J3; MGC14458;
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Species
Human
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Source
Baculovirus
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Tag
N-6*His;N-GST;N-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P11802-1 (M1-E303)&P24385
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Expression Region
P11802-1 (M1-E303)&P24385 (M1-I295)
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Molecular Weight
60.3 kDa&36.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDK4-CCND1 fusion protein is a significant focus of research due to its implication in various malignancies, particularly in human cancers such as melanoma and various types of sarcomas. The cyclin D1 (CCND1) gene regulates cell cycle progression and is critical for the transition from the G1 phase to the S phase, while cyclin-dependent kinase 4 (CDK4) functions as a key regulator in this process. The fusion of these two genes leads to the production of a hybrid protein that may exhibit abnormal regulatory functions, promoting uncontrolled cellular proliferation. This abnormal activity not only enhances the phosphorylation of retinoblastoma protein (Rb), bypassing crucial cell cycle checkpoints, but also disrupts normal cellular signaling pathways. As a result, understanding the structural and functional aspects of CDK4-CCND1 fusion proteins is essential for developing targeted therapeutic strategies, including small molecule inhibitors and immunotherapies. Furthermore, the study of this fusion protein offers insights into the molecular mechanisms underlying tumorigenesis and could lead to the identification of novel biomarkers for early detection and prognosis, thus improving patient outcomes. By elucidating the role of CDK4-CCND1 in cancer biology, researchers aim to contribute to the advancement of precision medicine approaches that specifically address the challenges posed by tumors harboring this fusion protein.
