Analytical Data
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基因名
PCBP1
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简介
PCBP1, a single-stranded nucleic acid binding protein with a specific affinity for oligo dC, is crucial for erythropoiesis when paired with PCBP2, potentially impacting mRNA splicing. In poliovirus infection, PCBP1 plays a vital role in initiating viral RNA replication alongside the viral protein 3CD, showcasing its multifunctional capacity in nucleic acid binding and involvement in fundamental cellular and viral processes. PCBP1 Protein, Human (His) is the recombinant human-derived PCBP1 protein, expressed by E. coli , with N-His labeled tag.
- Application
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别名
Poly(rC)-binding protein 1; Alpha-CP1; hnRNP E1; PCBP1
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15365
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表达区间
D2-C356
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氨基酸序列
DAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGC
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蛋白长度
Partial
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分子量
42 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PCBP1 (Poly(rC) binding protein 1) is an essential RNA-binding protein that plays a critical role in various cellular processes, including mRNA trafficking, translation, and stability. Its involvement in post-transcriptional regulation makes it a significant focus of research, especially in relation to viral infections and cancer. PCBP1 can interact with various RNA molecules and proteins, influencing gene expression and cellular responses to stress. Due to its multifunctional nature, researchers are interested in characterizing PCBP1 as a recombinant protein to explore its structure-function relationships and potential interactions with other biomolecules. The recombinant production of PCBP1 allows for detailed biochemical and biophysical studies, which can elucidate its mechanisms of action and potential as a therapeutic target. Understanding the role of PCBP1 in pathophysiological processes could pave the way for novel strategies in treating diseases where its function is disrupted, including viral pathogenesis and tumorigenesis. This has spurred interest in developing PCBP1-based assays and therapeutic interventions, making it a crucial component of ongoing studies in molecular biology and medicine.
