Analytical Data
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Gene name
AMSHLP
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简介
AMSHLP is a zinc metalloprotease that selectively cleaves "Lys-63" linked polyubiquitin chains and acts as a positive regulator of the TORC1 signaling pathway. It specifically deubiquitinates SESN2, disrupts its interaction with the GATOR2 complex and inhibits SESN2-mediated regulation of TORC1. AMSHLP Protein, Human (His) is the recombinant human-derived AMSHLP protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
STAMBP; STAM-binding protein; Associated molecule with the SH3 domain of STAM; Endosome-associated ubiquitin isopeptidase
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96FJ0
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Expression Region
D2-R436
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of AMSHLP (associated with the deubiquitinating enzyme AMSH and its homologs) has garnered increasing attention due to its critical role in the regulation of protein degradation and cellular signaling pathways. Ubiquitination is a key post-translational modification that labels proteins for degradation by the proteasome, thus controlling various cellular functions. As a deubiquitinating enzyme, AMSHLP reverses ubiquitination, thereby stabilizing target proteins and influencing their activity. This regulation is crucial in many physiological processes, including cell cycle progression, immune response, and DNA repair. Research has shown that dysregulation of AMSHLP and similar enzymes can lead to various diseases, including cancer and neurodegenerative disorders. The reconstitution of AMSHLP proteins allows scientists to explore their structural properties, enzymatic activity, and interactions with substrate proteins. Advanced techniques such as X-ray crystallography and mass spectrometry are utilized to understand the molecular mechanisms underlying AMSHLP function. Ultimately, studying AMSHLP and its reassembled forms contributes to the broader field of proteostasis and offers potential therapeutic targets for diseases associated with ubiquitin system malfunctions. This line of research emphasizes the significance of AMSHLP not just in basic biology but also in the development of novel strategies for disease treatment and prevention.
