Analytical Data
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Gene name
EIF3S5
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简介
The EIF3S5 protein is an important component of the eukaryotic translation initiation factor 3 (eIF-3) complex and plays a crucial role in various stages of protein synthesis initiation. It stimulates mRNA recruitment, scans for AUG recognition, and promotes disassembly and recycling of posttermination ribosomal complexes within the 43S preinitiation complex (43S PIC). EIF3S5 Protein, Human (GST) is the recombinant human-derived EIF3S5 protein, expressed by E. coli , with N-GST labeled tag.
- Application
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Alternative Names
EIF3F; Eukaryotic translation initiation factor 3 subunit F; eIF3f; Deubiquitinating enzyme eIF3f; Eukaryotic translation initiation factor 3 subunit 5; eIF-3-epsilon; eIF3 p47
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Species
Human
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Source
E. coli
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00303
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Expression Region
A2-L357
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Protein Length
Full Length of Mature Protein
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EIF3S5, a crucial component of the eukaryotic translation initiation factor 3 (eIF3) complex, plays a vital role in the regulation of protein synthesis and cellular function. It is involved in the recruitment of the ribosome to mRNA, thereby facilitating the initiation of translation. Disruptions in eIF3S5 expression have been implicated in various diseases, including cancer, highlighting its potential as a therapeutic target. Research on the recombinant protein EIF3S5 primarily focuses on understanding its structural characteristics and functional mechanisms. By producing EIF3S5 as a recombinant protein, scientists can perform biochemical assays, elucidate interaction dynamics with other translation factors, and investigate its role within the entire eIF3 complex. Advances in recombinant protein technologies have enabled more efficient purification and characterization of EIF3S5, paving the way for high-throughput screening of small molecules that can modulate its activity. Additionally, the study of EIF3S5 may reveal novel insights into the translational control mechanisms that underpin disease states, contributing to the development of targeted therapies aimed at modulating protein synthesis in pathological conditions. Overall, the study of EIF3S5 recombinant protein is essential for unraveling the complexities of translation initiation and its implications in health and disease.
