Analytical Data
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Gene name
UDG
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简介
UDG Protein, an uracil-DNA glycosylase, plays a pivotal role in DNA repair by excising uracil residues, addressing misincorporation or deamination-induced uracil. This activity is crucial for genomic integrity, preventing mutagenesis. UDG's precision in recognizing and excising uracil underscores its significance in cellular DNA repair mechanisms and fidelity. UDG Protein, E.coli (His) is the recombinant E. coli-derived UDG protein, expressed by E. coli , with His labeled tag. UDG Protein, E.coli (His), has molecular weight of ~25.7 kDa.
- Application
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Species
E.coli
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Source
E. coli
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Tag
His
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Purity
Greater than 90% as determined by SDS-PAGE.
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AA Sequence
MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDVFNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPGFTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVVFLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPIDWMPVLPAESE
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Molecular Weight
25.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UDG (Uracil-DNA Glycosylase) is an enzyme that plays a crucial role in the base excision repair pathway, specifically in the recognition and removal of uracil from DNA. This process is essential for maintaining genomic stability, as uracil can arise from the deamination of cytosine or be incorporated into DNA during replication. Impairments in UDG function have been implicated in various diseases, including cancer, making it an important target for therapeutic interventions. Researchers have been investigating the structure and function of UDG to understand its mechanism of action at the molecular level. The recombinant expression of UDG has facilitated detailed studies on its enzymatic properties, substrate specificity, and interaction with DNA. Furthermore, UDG's potential applications in biotechnology, such as its use in gene editing and synthetic biology, have spurred interest in optimizing its activity and stability. As studies continue to reveal the complexities of UDG's role in DNA repair and its broader biological implications, it remains a significant focus of research aimed at developing novel strategies for disease treatment and prevention.
