Analytical Data
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Gene name
RIOK2
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简介
RIOK2 is an important serine/threonine protein kinase that is critical in the final cytoplasmic maturation phase of the 40S ribosomal subunit. It promotes the export of 40S preribosomal particles from the nucleus to the cytoplasm, which is essential for ribosome biogenesis. RIOK2 Protein, Human (sf9, His, GST) is the recombinant human-derived RIOK2 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
- Application
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Alternative Names
RIOK2; Serine/threonine-protein kinase RIO2; RIO kinase 2
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Species
Human
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Source
Baculovirus
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Tag
N-8*His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BVS4
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Expression Region
G2-E552
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AA Sequence
GKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSAVSCSTIPPELVKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE
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Protein Length
Partial
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Molecular Weight
90.8 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RIOK2, a member of the RIO kinase family, is an evolutionarily conserved protein involved in various cellular processes, including ribosome biogenesis, cell proliferation, and stress response. Initial studies indicated that RIOK2 plays a crucial role in the production of ribosomal RNA and the assembly of ribosomal subunits, thus influencing protein synthesis and cell growth. Recent research has highlighted its involvement in cancer biology, suggesting that alterations in RIOK2 expression or function may contribute to tumorigenesis and cancer progression. Furthermore, RIOK2 has been implicated in cellular stress responses, particularly in relation to the unfolded protein response and oxidative stress. These functions collectively underscore the importance of RIOK2 in maintaining cellular homeostasis. Given its multifaceted roles, RIOK2 has emerged as a potential therapeutic target, prompting investigations into the mechanisms governing its function and regulation. Understanding the structural and functional dynamics of RIOK2 through recombinant protein studies may provide insights into its biological significance and therapeutic potential, ultimately contributing to the development of novel strategies for cancer treatment and other diseases associated with dysregulated cellular functions.
