Analytical Data
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基因名
WEE2
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简介
WEE2 protein is an oocyte-specific tyrosine kinase that plays an important role in meiosis during prophase I and metaphase II. It maintains meiotic arrest at the germinal vesicle (GV) stage by phosphorylating CDK1 at 'Tyr-15', inhibiting CDK1 activity and preventing reentry. WEE2 Protein, Human (sf9, His, GST) is the recombinant human-derived WEE2 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
- Application
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别名
WEE2; Wee1-like protein kinase 2; Wee1-like protein kinase 1B; Wee1B kinase
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种属
Human
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表达系统
Baculovirus
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标签
N-8*His;N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0C1S8
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表达区间
D2-H567
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氨基酸序列
DDKDIDKELRQKLNFSYCEETEIEGQKKVEESREASSQTPEKGEVQDSEAKGTPPWTPLSNVHELDTSSEKDKESPDQILRTPVSHPLKCPETPAQPDSRSKLLPSDSPSTPKTMLSRLVISPTGKLPSRGPKHLKLTPAPLKDEMTSLALVNINPFTPESYKKLFLQSGGKRKIRGDLEEAGPEEGKGGLPAKRCVLRETNMASRYEKEFLEVEKIGVGEFGTVYKCIKRLDGCVYAIKRSMKTFTELSNENSALHEVYAHAVLGHHPHVVRYYSSWAEDDHMIIQNEYCNGGSLQAAISENTKSGNHFEEPKLKDILLQISLGLNYIHNSSMVHLDIKPSNIFICHKMQSESSGVIEEVENEADWFLSANVMYKIGDLGHATSINKPKVEEGDSRFLANEILQEDYRHLPKADIFALGLTIAVAAGAESLPTNGAAWHHIRKGNFPDVPQELSESFSSLLKNMIQPDAEQRPSAAALARNTVLRPSLGKTEELQQQLNLEKFKTATLERELREAQQAQSPQGYTHHGDTGVSGTHTGSRSTKRLVGGKSARSSSFTSGEREPLH
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蛋白长度
Partial
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分子量
90.5 KDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
WEE2 is a protein that plays a crucial role in the regulation of the cell cycle, particularly in oocyte maturation and fertility. Research has shown that WEE2, a member of the WEE family of serine/threonine kinases, is involved in the process of oocyte meiotic progression by modulating the activity of cyclin-dependent kinases (CDKs). A better understanding of WEE2's function not only sheds light on fundamental aspects of reproductive biology but also has potential implications for addressing fertility issues in both humans and animals. Studies have indicated that alterations in WEE2 expression can lead to defects in oocyte development and maturation, resulting in impaired fertility. Consequently, there is a growing interest in characterizing WEE2, including its structure, function, and regulatory mechanisms, to develop strategies for enhancing fertility and improving outcomes in assisted reproductive technologies. Furthermore, understanding the interplay between WEE2 and other molecular players in the oocyte maturation process could provide insights into novel therapeutic targets for fertility treatments. The ongoing exploration of WEE2's role plants the groundwork for advancements in reproductive science and offers hope for those facing challenges with conception.
