Analytical Data
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Gene name
SUMO1
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简介
SUMO1 Protein is a member of the SUMO (small ubiquitin-like modifier) protein family. SUMO1 binds to target proteins as part of a post-translational modification system. It is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. SUMO1 also regulates the function of several proteins via non-covalent interactions. SUMO1 Protein, Human (His) is the recombinant human-derived SUMO1 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized SUMO1 at 2 μg/mL (100 μL/well) can bind SUMO1 Antibody (HY-P80351).The ED50 for this effect is 3.853 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized SUMO1 at 2 μg/mL (100 μL/well) can bind SUMO1 Antibody (HY-P80351).The ED50 for this effect is 3.853 ng/mL.
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
AAH66306
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Expression Region
M1-V101
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Protein Length
Full Length
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Molecular Weight
17-19 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SUMO1 (Small Ubiquitin-like Modifier 1) is a key protein involved in the post-translational modification known as SUMOylation, a critical regulatory mechanism that influences various cellular processes, including gene expression, protein stability, and cellular localization. SUMOylation can affect numerous substrates, playing significant roles in cellular responses to stress, DNA repair, and the regulation of the cell cycle. Aberrant SUMOylation has been implicated in various diseases, including cancer and neurodegenerative disorders, highlighting the importance of understanding SUMO1's structure and function. Recent studies have focused on the biochemical properties of SUMO1 and its interactions with other cellular proteins, aiming to elucidate the molecular mechanisms underlying its role in cell physiology. Recombinant SUMO1 proteins are increasingly used in research to investigate the complexities of SUMOylation pathways and to develop potential therapeutic strategies for diseases linked to its dysregulation. By producing functional SUMO1 through recombinant methods, researchers can examine its effects in vitro and in vivo, helping to advance our understanding of its biological significance and potential as a therapeutic target.
