Analytical Data
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Gene name
GDF-9
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简介
The GDF-9 protein is critical for ovarian folliculogenesis, promoting primordial follicle development and stimulating granulosa cell proliferation. It induces cell cycle transition, upregulates CCND1 and CCNE1 expression, and phosphorylates RB1. GDF-9 Protein, Human (GST) is the recombinant human-derived GDF-9 protein, expressed by E. coli , with N-GST labeled tag.
- Application
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Alternative Names
GDF9; Growth/differentiation factor 9; GDF-9
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Species
Human
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Source
E. coli
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60383
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Expression Region
G320-R454
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Protein Length
Full Length of Mature Protein
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Molecular Weight
40-45 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GDF-9 (Growth Differentiation Factor 9) is a member of the transforming growth factor-beta (TGF-β) superfamily, primarily involved in the regulation of ovarian follicle development and fertility in mammals. The protein plays a crucial role in various biological processes, including cell growth, differentiation, and apoptosis. Research has shown that GDF-9 is essential for oocyte maturation and the development of ovarian somatic cells, making it a pivotal factor in female reproductive biology. Investigations into GDF-9 recombinant proteins have gained attention due to their potential therapeutic applications in treating infertility and improving assisted reproductive technologies. The expression and functional analysis of GDF-9 can provide insights into its mechanisms of action and its interactions with other growth factors and hormones in the reproductive system. Moreover, recombinant GDF-9 can be used in animal models to explore its role in folliculogenesis and oogenesis, thereby advancing our understanding of various reproductive disorders. Overall, the study of GDF-9 recombinant proteins not only contributes to fundamental reproductive biology but also holds promise for application in clinical settings, underscoring the importance of ongoing research in this area.
