Analytical Data
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基因名
ERK1
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简介
ERK1 protein is an important serine/threonine kinase in the MAP kinase pathway and cooperates with MAPK1/ERK2 to activate the MAPK/ERK cascade. Stimulated by KIT and KITLG/SCF, this pathway plays a key role in cell growth, adhesion, survival, and differentiation. ERK1 Protein, Human (GST) is the recombinant human-derived ERK1 protein, expressed by E. coli , with N-GST labeled tag.
- Application
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种属
Human
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表达系统
E. coli
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标签
N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P27361
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表达区间
A2-P379
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蛋白长度
Full Length of Mature Protein
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分子量
69.3 KDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ERK1 (Extracellular signal-Regulated Kinase 1) is a critical component of the mitogen-activated protein kinase (MAPK) signaling pathway, playing a vital role in various cellular processes such as proliferation, differentiation, and survival. Research has shown that ERK1 is activated in response to growth factors and stress stimuli, leading to the phosphorylation of downstream substrates that ultimately regulate gene expression. Understanding the function and regulation of ERK1 is essential for elucidating its role in cancer biology, as aberrant ERK signaling is often implicated in tumorigenesis and disease progression. The production of recombinant ERK1 proteins has been crucial for studying its biochemical properties, post-translational modifications, and interactions with other cellular molecules. Moreover, by generating ERK1 in a controlled environment, researchers can perform detailed assays to uncover the nuances of its activity and regulation, paving the way for potential therapeutic interventions in diseases characterized by dysregulated MAPK signaling. Thus, the study of recombinant ERK1 proteins not only enhances our comprehension of fundamental cellular mechanisms but also potentially provides insights for drug development and targeted therapies in oncology.
