Analytical Data
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基因名
NEDD4L
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简介
The NEDD4L protein is an E3 ubiquitin protein ligase that regulates multiple signaling pathways, including autophagy, innate immunity, and DNA repair. NEDD4L Protein, Human (His) is the recombinant human-derived NEDD4L protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
NEDD4L; E3 ubiquitin-protein ligase NEDD4-like; HECT-type E3 ubiquitin transferase NED4L; NEDD4.2; Nedd4-2
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96PU5
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表达区间
A2-D975
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蛋白长度
Full Length of Mature Protein
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分子量
113.9 KDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NEDD4L, a member of the NEDD4 family of E3 ubiquitin ligases, has garnered considerable attention in recent years due to its crucial role in regulating various cellular processes, including protein degradation, trafficking, and signaling pathways. It is involved in the ubiquitin-proteasome system, which is essential for maintaining cellular homeostasis by controlling the degradation of proteins tagged for destruction. Dysregulation of NEDD4L has been implicated in several human diseases, including hypertension, cancer, and metabolic disorders, highlighting its potential as a therapeutic target. Research into recombinant NEDD4L proteins aims to better understand its functional mechanisms and interactions at the molecular level. By producing and characterizing recombinant NEDD4L, scientists can elucidate its structure-function relationships, identify binding partners, and explore its enzymatic activity. Ultimately, this knowledge may facilitate the development of NEDD4L-targeted drugs and provide insights into the broader implications of ubiquitination in health and disease. Furthermore, recombinant proteins serve as valuable tools for high-throughput screening and in vivo studies, thereby enhancing our understanding of NEDD4L's role in various biological contexts.
