Analytical Data
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Gene name
CDK8-CCNC-MED12
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简介
The CDK8-CCNC-MED12 protein is an important component of the mediator complex, acting as a bridge for gene transcription regulation, transmitting information from gene-specific regulatory factors to RNA polymerase II. It is recruited to the promoter, assembles a preinitiation complex, and promotes transcription. CDK8-CCNC-MED12 Protein, Human (sf9, GST, FLAG, His) is the recombinant human-derived CDK8-CCNC-MED12 protein, expressed by sf9 insect cells , with N-6*His, N-Flag, N-GST labeled tag.
- Application
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Species
Human
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Source
Baculovirus
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Tag
N-Flag;N-GST;N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P49336-1 (M1-Y464)&P24863-1 (M1-S283)&Q93074-1
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Expression Region
P49336-1 (M1-Y464)&P24863-1 (M1-S283)&Q93074-1 (M1-D100)
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Molecular Weight
79.8 kDa&36 kDa&12.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The CDK8-CCNC-MED12 protein complex plays a crucial role in the regulation of transcription in eukaryotic cells, functioning as a part of the Mediator complex, which serves as a critical link between gene-specific regulatory proteins and the general transcription machinery. Recent studies have highlighted the significance of this complex in various cellular processes, including cell proliferation, differentiation, and response to stress. Dysregulation of the CDK8-CCNC-MED12 complex has been implicated in several diseases, including cancer, where it may affect the expression of oncogenes and tumor suppressor genes. Research has aimed to elucidate the structural and functional properties of the reconstituted CDK8-CCNC-MED12 complex to understand its mechanistic roles better. By studying this complex, scientists hope to uncover novel therapeutic targets for diseases associated with transcriptional dysregulation. Understanding the interactions within this complex offers insights into the regulatory networks governing gene expression and may facilitate the development of small-molecule inhibitors that can modulate its activity in pathological conditions. This area of research is increasingly relevant as it combines insights from molecular biology, biochemistry, and drug discovery to address significant health challenges.
