Analytical Data
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Gene name
Can f 4
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简介
Can f 4 Protein, Canine (HEK293, His), an IgE-binding dog dander protein, is an important respiratory allergen. Can f 4 Protein is a dog allergen of the lipocalin family[1]. Can f 4 Protein, Canine (HEK293, His) is the recombinant canine-derived Can f 4 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Alternative Names
Allergen Can f 4
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Species
Canine
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
NP_001177855.1
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Expression Region
Q17-E174
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Protein Length
Full Length of Mature Protein
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Molecular Weight
19 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Can f 4 is a major allergen derived from domestic dogs, specifically identified as a protein expressed in their saliva, skin, and urine. As a significant contributor to pet allergy, Can f 4 has garnered considerable attention within the fields of immunology and allergology. The increase in pet ownership globally has led to a rise in allergic reactions among individuals sensitive to dog allergens, highlighting the need for effective diagnostic and therapeutic strategies. Studies on Can f 4's structure and function provide insight into its role in the allergenic response. Understanding the molecular basis of its allergenicity can facilitate the development of targeted immunotherapies and improve methods for allergen detection. Additionally, the recombinant production of Can f 4 allows for large-scale availability of the protein, which can be utilized in research and clinical settings. By exploring the IgE-binding properties of Can f 4, researchers aim to uncover potential cross-reactivity with other allergens, ultimately contributing to a deeper understanding of allergic mechanisms. This research not only aids in improving allergy management but also enhances the quality of life for individuals affected by pet allergies.
