Analytical Data
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Gene name
BMP-13/GDF-6
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简介
The BMP-13/GDF-6 protein is a multifunctional growth factor that plays a critical role in retinal development, control of apoptosis, and dorsoventral positional information. It is critical for bone and joint development in various anatomical regions, influencing species-specific skeletal changes and contributing to the evolution of unique anatomical features. BMP-13/GDF-6 Protein, Mouse is the recombinant mouse-derived BMP-13/GDF-6 protein, expressed by E. coli , with tag free.
- Application
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Biological Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.85-5 µg/mL. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 this effect is 1.808 μg/mL, corresponding to a specific activity is 553.097 units/mg.
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Alternative Names
Growth/differentiation factor 6; Gdf6; GDF-6; Bone morphogenetic protein 13; BMP-13; Growth/differentiation factor 16; Growth Differentiation Factor 6
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Species
Mouse
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P43028
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Expression Region
T335-R454
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Protein Length
Full Length of Mature Protein
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Molecular Weight
14 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BMP-13 (Bone Morphogenetic Protein-13), also known as GDF-6 (Growth and Differentiation Factor-6), is a member of the transforming growth factor-beta (TGF-β) superfamily. It plays a crucial role in various biological processes, including embryonic development, bone formation, and tissue regeneration. The significance of BMP-13/GDF-6 in developmental biology has garnered attention due to its potential applications in regenerative medicine and orthopedic therapies. Research has indicated that BMP-13 promotes the differentiation of mesenchymal stem cells into chondrocytes and osteoblasts, thereby facilitating cartilage and bone tissue engineering. Furthermore, its regulatory effects on cellular signaling pathways make it a vital player in the maintenance of bone homeostasis and repair mechanisms. Due to these critical functions, recombinant BMP-13/GDF-6 proteins have been extensively studied for their therapeutic potential in treating conditions like osteoarthritis, osteoporosis, and fractures. Understanding the molecular mechanisms underlying the activity of BMP-13/GDF-6 is essential for developing innovative treatments that harness its regenerative properties. Recent advancements in recombinant protein technologies have enabled the production of bioactive BMP-13/GDF-6, paving the way for preclinical and clinical investigations. As a result, the exploration of BMP-13/GDF-6 in experimental models and clinical settings continues to expand, offering promising insights into enhancing musculoskeletal health and improving outcomes for patients with skeletal disorders.
