Analytical Data
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基因名
PRAK
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简介
PRAK/MAPKAPK5 protein, a serine/threonine kinase, responds to cellular stress and pro-inflammatory cytokines and acts as a tumor suppressor through MAP kinases (including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta) activated by phosphorylation. Initially located in the nucleus, upon activation, it moves to the cytoplasm and phosphorylates the heat shock protein HSP27. PRAK Protein, Human (sf9, Strep) is the recombinant human-derived PRAK protein, expressed by Sf9 insect cells, with C-Strep labeled tag.
- Application
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种属
Human
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表达系统
Baculovirus
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标签
C-Strep
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
NP_003659
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表达区间
M1-Q471
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蛋白长度
Full Length
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分子量
35-45 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PRAK (p53-regulated protein kinase) is a crucial serine/threonine kinase that plays a significant role in cellular responses to stress and has been implicated in various physiological processes, including cell survival, differentiation, and apoptosis. Originally identified as a target of the tumor suppressor protein p53, PRAK is involved in the activation of the MKK3/MKK6-p38 MAPK signaling pathway, which modulates the cellular response to stressors such as DNA damage, inflammation, and oxidative stress. Research on PRAK has gained momentum due to its potential implications in cancer biology, where its regulatory functions can influence tumor progression and treatment responses. Additionally, understanding the precise molecular mechanisms through which PRAK exerts its effects can facilitate the development of targeted therapies that may enhance cancer treatment efficacy. Recent studies have also highlighted PRAK’s involvement in other diseases, including neurodegenerative disorders and metabolic syndromes, suggesting broader biological significance beyond cancer. Given its multifaceted roles, the study of PRAK and its interactions within cellular signaling networks is essential for elucidating its contributions to both health and disease, making it a compelling target for future therapeutic interventions and biomarker development.
