Analytical Data
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Gene name
Sphingomyelin Synthase 2/SGMS2
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简介
The sphingomyelin synthase 2 (SGMS2) protein plays a crucial role in plasma membrane sphingomyelin synthesis. It catalyzes the reversible transfer of the phosphocholine moiety in sphingomyelin biosynthesis to form ceramide phosphocholine. Sphingomyelin Synthase 2/SGMS2 Protein, Human (HEK293, Fc) is the recombinant human-derived Sphingomyelin Synthase 2/SGMS2 protein, expressed by HEK293 , with N-mFc labeled tag.
- Application
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Alternative Names
Phosphatidylcholine:ceramide cholinephosphotransferase 2; Sphingomyelin synthase 2; SGMS2; SMS2
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Species
Human
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Source
HEK293
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Tag
N-mFc
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q8NHU3
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Expression Region
M1-T79
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Protein Length
Partial
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Molecular Weight
35.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Sphingomyelin Synthase 2 (SGMS2) is a key enzyme involved in sphingolipid metabolism, specifically responsible for catalyzing the synthesis of sphingomyelin from ceramide and phosphatidylcholine. Sphingomyelin is a crucial lipid component of cell membranes, playing vital roles in membrane structure, signaling, and cellular interactions. Aberrant sphingomyelin metabolism has been linked to various pathological conditions, including cancer, neurodegenerative disorders, and cardiovascular diseases. Recent research highlights the significance of SGMS2 in regulating cellular responses and its potential as a therapeutic target. The recombinant expression and characterization of SGMS2 are essential for understanding its biochemical properties and functionality. Utilizing recombinant protein techniques allows for the production of SGMS2 in a controlled environment, facilitating the study of its enzymatic activity, substrate specificity, and role in sphingolipid biosynthesis. Furthermore, structural studies of SGMS2 could reveal insights into the enzyme's mechanism and help design inhibitors or modulators that could be used in therapeutic applications. Thus, the investigation of recombinant SGMS2 not only enhances our understanding of sphingolipid metabolism but also holds promise for advancing drug development in related diseases.
