Analytical Data
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基因名
ECE-2
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简介
The ECE-2 protein plays a crucial role in vascular regulation by catalyzing the conversion of large endothelin-1 to endothelin-1, helping to regulate blood pressure and vascular tone. Furthermore, ECE-2 exhibits potential methyltransferase activity and may be involved in amyloid-β processing, suggesting that this enzyme has a broader impact on cellular function than its established role in endothelin processing. ECE-2 Protein, Human (HEK293, hFc) is the recombinant human-derived ECE-2 protein, expressed by HEK293 , with N-hFc labeled tag.
- Application
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别名
EEF1AKMT4-ECE2 readthrough transcript protein; EEF1AKMT4-ECE2; ECE2
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种属
Human
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表达系统
HEK293
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标签
N-hFc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0DPD8-1
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表达区间
G199-W883
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蛋白长度
Partial
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分子量
120-130 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The ECE-2 (Endothelin-Converting Enzyme 2) is an essential enzyme involved in the processing of endothelin-1, a potent vasoconstrictor that plays a critical role in cardiovascular regulation and various pathological conditions. Understanding the structure and function of ECE-2 is pivotal due to its implications in diseases such as hypertension, heart failure, and chronic kidney disease. Previous research has indicated that alterations in ECE-2 activity can lead to an imbalance in endothelin levels, significantly impacting vascular tone and fluid balance in the body. The study of ECE-2 also holds therapeutic potential; specifically, it can serve as a target for the development of novel antihypertensive drugs that may help mitigate the adverse effects associated with endothelin overactivity. Recent advances in recombinant DNA technology have enabled the production of ECE-2 fusion proteins, allowing for more detailed biochemical studies and the exploration of its inhibition mechanisms. Furthermore, these studies aim to elucidate the enzyme's role in various signaling pathways and its potential interactions with other proteins within the endothelin system. By employing techniques such as structural biology and molecular dynamics simulations, researchers are striving to gain insights that can lead to the development of targeted therapies and better understand the enzyme's function in health and disease. Consequently, ECE-2 represents a critical focus in cardiovascular research, highlighting the need for ongoing investigation into its biological significance and therapeutic applications.
