Analytical Data
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Gene name
ECE-2
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简介
The ECE-2 protein plays a crucial role in vascular regulation by catalyzing the conversion of large endothelin-1 to endothelin-1, helping to regulate blood pressure and vascular tone. Furthermore, ECE-2 exhibits potential methyltransferase activity and may be involved in amyloid-β processing, suggesting that this enzyme has a broader impact on cellular function than its established role in endothelin processing. ECE-2 Protein, Human (HEK293, hFc) is the recombinant human-derived ECE-2 protein, expressed by HEK293 , with N-hFc labeled tag.
- Application
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Alternative Names
EEF1AKMT4-ECE2 readthrough transcript protein; EEF1AKMT4-ECE2; ECE2
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Species
Human
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Source
HEK293
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Tag
N-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0DPD8-1
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Expression Region
G199-W883
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Protein Length
Partial
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Molecular Weight
120-130 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The ECE-2 (Endothelin-Converting Enzyme 2) is an essential enzyme involved in the processing of endothelin-1, a potent vasoconstrictor that plays a critical role in cardiovascular regulation and various pathological conditions. Understanding the structure and function of ECE-2 is pivotal due to its implications in diseases such as hypertension, heart failure, and chronic kidney disease. Previous research has indicated that alterations in ECE-2 activity can lead to an imbalance in endothelin levels, significantly impacting vascular tone and fluid balance in the body. The study of ECE-2 also holds therapeutic potential; specifically, it can serve as a target for the development of novel antihypertensive drugs that may help mitigate the adverse effects associated with endothelin overactivity. Recent advances in recombinant DNA technology have enabled the production of ECE-2 fusion proteins, allowing for more detailed biochemical studies and the exploration of its inhibition mechanisms. Furthermore, these studies aim to elucidate the enzyme's role in various signaling pathways and its potential interactions with other proteins within the endothelin system. By employing techniques such as structural biology and molecular dynamics simulations, researchers are striving to gain insights that can lead to the development of targeted therapies and better understand the enzyme's function in health and disease. Consequently, ECE-2 represents a critical focus in cardiovascular research, highlighting the need for ongoing investigation into its biological significance and therapeutic applications.
