Analytical Data
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基因名
WISP1/CCN4
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简介
WISP1/CCN4 Protein, a member of the CCN family, is involved in cellular processes such as cell proliferation, migration, and differentiation.It is implicated in various diseases, including cancer and fibrosis.The potential therapeutic targeting of WISP1/CCN4 Protein and its role in disease pathogenesis make it a subject of interest in biomedical research.WISP1/CCN4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived WISP1/CCN4 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by its ability to inhibit the cell growth of DU145 human prostate cancer cells. The ED50 for this effect is 0.5-3.0 µg/mL. Measured by its ability to inhibit the cell growth of DU145 human prostate cancer cells. The ED50 for this effect is 0.9133 µg/mL, corresponding to a specific activity is 1094.930 U/mg.
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别名
ELM-1; WISP-1; CCN4; WISP1c; WISP1i; WISP1tc
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种属
Mouse
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
O54775
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表达区间
T23-N367
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氨基酸序列
TALSPTPTTMTFTPAPLEETTTRPEFCKWPCECPQSPPRCPLGVSLITDGCECCKICAQQLGDNCTEAAICDPHRGLYCDYSGDRPRYAIGVCAQVVGVGCVLDGVRYTNGESFQPNCRYNCTCIDGTVGCTPLCLSPRPPRLWCRQPRHVRVPGQCCEQWVCDDDARRPRQTALLDTRAFAASGAVEQRYENCIAYTSPWSPCSTTCGLGISTRISNVNARCWPEQESRLCNLRPCDVDIQLHIKAGKKCLAVYQPEEATNFTLAGCVSTRTYRPKYCGVCTDNRCCIPYKSKTISVDFQCPEGPGFSRQVLWINACFCNLSCRNPNDIFADLESYPDFEEIAN
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蛋白长度
Full Length of Mature Protein
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分子量
55-75 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
WISP1, also known as CCN4, is a member of the CCN family of secreted proteins, which play pivotal roles in various biological processes, including cell proliferation, differentiation, and extracellular matrix regulation. Research has identified WISP1/CCN4 as a critical factor involved in the regulation of fibrotic diseases, wound healing, and cancer progression. It functions through complex signaling pathways, interacting with various cell surface receptors and modulating intracellular signaling cascades. The dysregulation of WISP1 has been implicated in several pathological conditions, including fibrosis and malignancies, making it a potential therapeutic target. Studies have demonstrated that enhanced expression of WISP1 correlates with tumor growth and metastasis, highlighting its role as a mediator of the tumor microenvironment. Considering its significance in health and disease, the recombinant production of WISP1/CCN4 protein has gained attention for enabling functional studies and potential therapeutic applications. By utilizing recombinant DNA technology, researchers can produce large quantities of this protein to explore its biological functions, investigate its mechanisms of action in various cellular contexts, and assess its potential as a biomarker or therapeutic agent in fibrotic diseases and cancer. The ongoing research into WISP1/CCN4 promises to deepen our understanding of its role in cellular processes and may facilitate the development of novel strategies for the treatment of related diseases.
