Analytical Data
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Gene name
GDF-15
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简介
GDF-15 Protein, a key regulator of food intake and energy balance, binds to its receptor GFRAL, activating neurons in the brainstem and forming an 'emergency circuit' for feeding responses during stress. Additionally, GDF-15 inhibits growth hormone signaling on hepatocytes, existing as a disulfide-linked homodimer. Interacting with GFRAL, it acts as a ligand, mediating GDF15 internalization and cellular signaling through RET. GDF-15 Protein, Cynomolgus (His) is the recombinant cynomolgus-derived GDF-15 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β
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Species
Cynomolgus
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Source
E. coli
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Tag
N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
G7PWZ3
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Expression Region
R193-V308
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Protein Length
Full Length of Mature Protein
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Molecular Weight
15-18 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GDF-15 (Growth Differentiation Factor 15) is a member of the transforming growth factor-beta (TGF-β) superfamily and has emerged as a significant biomarker and therapeutic target in various diseases, including cancer, cardiovascular diseases, and metabolic disorders. Elevated levels of GDF-15 correlate with disease severity, making it a potential indicator of poor prognosis. Its role in regulating inflammation, apoptosis, and tissue repair further underscores its biological importance. Recent studies have highlighted its involvement in the cellular response to stress and injury, suggesting a protective mechanism that could be exploited for therapeutic purposes. As a result, the production of recombinant GDF-15 protein has garnered interest for both research and clinical applications. This involves utilizing advanced recombinant DNA technology to produce GDF-15 in suitable expression systems, allowing for the exploration of its physiological roles and potential as a therapeutic agent. Additionally, understanding the molecular pathways influenced by GDF-15 can provide insights into its mechanisms of action and aid in the development of targeted therapies. Hence, ongoing research revolves around refining its production, characterizing its biological functions, and evaluating its efficacy in preclinical and clinical settings, paving the way for innovative approaches to manage various diseases.
