Analytical Data
-
Gene name
BLM
- Application
-
Alternative Names
(Bloom syndrome protein)(DNA helicase, RecQ-like type 2)(RecQ2)(RecQ protein-like 3)
-
Species
Human
-
Source
E. coli
-
Tag
N- His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P54132
-
Expression Region
877-1024aa
-
Molecular Weight
23.0 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BLM (Bloom syndrome protein) is a crucial member of the RecQ helicase family, playing a significant role in maintaining genomic stability and preventing cancer. Mutations in the BLM gene lead to Bloom syndrome, a rare genetic disorder characterized by growth deficiencies, immunodeficiency, and a predisposition to various cancers. Given its pivotal role in DNA repair mechanisms, BLM helicase is essential for processes such as replication fork stabilization, resolution of DNA structures, and the maintenance of telomeres. Research on BLM recombinant proteins has gained momentum as scientists seek to understand its functional dynamics in DNA metabolism and repair pathways. By producing recombinant BLM proteins, researchers aim to elucidate the intricate mechanisms underlying its helicase activity and interactions with other key proteins involved in genomic maintenance. Furthermore, investigating post-translational modifications and structural characteristics of BLM can provide insights into how its activity is regulated in cellular contexts. The ultimate goal of these studies not only relates to understanding Bloom syndrome at a molecular level but also holds potential therapeutic implications for cancer treatment, as targeted strategies could emerge from a better understanding of the biological pathways driven by BLM helicase. Overall, the exploration of recombinant BLM proteins is critical for advancing our knowledge of DNA repair mechanisms and developing novel interventions for diseases linked to genomic instability.
