Analytical Data
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基因名
BLM
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简介
BLM is an ATP-dependent DNA helicase that can unwind single- and double-stranded DNA in the 3'-5' direction and actively participates in the DNA replication and repair process. Notably, BLM plays a critical role in 5' end resection during double-strand break repair, recruiting DNA2 to cleave 5'-ssDNA. BLM Protein, Mouse (His-Myc) is the recombinant mouse-derived BLM protein, expressed by E. coli , with N-10*His, C-Myc labeled tag.
- Application
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别名
BlmBloom syndrome protein homolog; mBLM; EC 3.6.4.12; RecQ helicase homolog
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种属
Mouse
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表达系统
E. coli
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标签
N-10*His;C-Myc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O88700
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表达区间
I684-Q859
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BLM (Bloom syndrome protein) is a crucial member of the RecQ helicase family, playing a significant role in maintaining genomic stability and preventing cancer. Mutations in the BLM gene lead to Bloom syndrome, a rare genetic disorder characterized by growth deficiencies, immunodeficiency, and a predisposition to various cancers. Given its pivotal role in DNA repair mechanisms, BLM helicase is essential for processes such as replication fork stabilization, resolution of DNA structures, and the maintenance of telomeres. Research on BLM recombinant proteins has gained momentum as scientists seek to understand its functional dynamics in DNA metabolism and repair pathways. By producing recombinant BLM proteins, researchers aim to elucidate the intricate mechanisms underlying its helicase activity and interactions with other key proteins involved in genomic maintenance. Furthermore, investigating post-translational modifications and structural characteristics of BLM can provide insights into how its activity is regulated in cellular contexts. The ultimate goal of these studies not only relates to understanding Bloom syndrome at a molecular level but also holds potential therapeutic implications for cancer treatment, as targeted strategies could emerge from a better understanding of the biological pathways driven by BLM helicase. Overall, the exploration of recombinant BLM proteins is critical for advancing our knowledge of DNA repair mechanisms and developing novel interventions for diseases linked to genomic instability.
