Analytical Data
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Gene name
NAD kinase/NADK
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简介
NAD kinase (NADK) is located in cytosol, catalyzing the transfer of a phosphate group from ATP to NAD to generate NADP. NADK contributes to cellular redox homeostasis, insulin secretion, cancer regulating, heart disease, anabolic and catabolic processes, neuronal development, and aging. The molecular mechanisms that regulate NADK include protein-protein interaction and post-translational modifications, such as phosphorylation and acetylation including Akt, CaMK and PKC pathways. NAD kinase/NADK Protein, Human (His) is the recombinant human-derived NAD kinase/NADK protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
rHuNAD kinase/NADK, His; NAD Kinase; Poly(P)/ATP NAD Kinase; NADK
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95544-1
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Expression Region
S64-G446
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AA Sequence
SLHGPCPVTTFGPKACVLQNPQTIMHIQDPASQRLTWNKSPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEKKVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGENGSQAAGLDMDVGKQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQAHFEEEEEEEEEG
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Protein Length
Partial
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Molecular Weight
49.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NAD kinase (NADK) is a crucial enzyme involved in the biosynthesis and homeostasis of nicotinamide adenine dinucleotide (NAD), a vital coenzyme that participates in numerous cellular processes, including metabolism, DNA repair, and redox reactions. The generation of NADP+ from NAD+ is catalyzed by NADK, linking NAD metabolism with the integrity of cellular redox state and various signaling pathways. Research on recombinant NADK proteins has gained significant attention due to their potential applications in biotechnology and medicine. Understanding the structure and function of NADK is essential not only for elucidating its role in cellular physiology but also for exploring its implications in various diseases, such as cancer and neurodegenerative disorders, where NAD metabolism is often disrupted. Advances in recombinant DNA technology allow for the production of high-purity NADK proteins, which can be used for biochemical assays, structural analyses, and as targets for drug discovery. Furthermore, the engineering of NADK has potential for metabolic engineering applications in industrial microbiology, where enhanced NADP+ levels can optimize the production of biofuels and biochemicals. Thus, researching recombinant NADK not only broadens our understanding of its enzymatic mechanisms but also holds promise for innovative therapeutic and biotechnological advancements.
