Analytical Data
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Gene name
PHPT1
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简介
The PHPT1 protein acts as a phosphohistidine phosphatase, catalyzing the dephosphorylation of phosphohistidine residues. This unique enzymatic activity makes PHPT1 a regulator of histidine phosphorylation events, suggesting that it is involved in signaling pathways or cellular processes that are critical for phosphohistidine modification. PHPT1 Protein, Human (P.pastoris, His) is the recombinant human-derived PHPT1 protein, expressed by P. pastoris , with N-His labeled tag.
- Application
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Alternative Names
14kDa phosphohistidine phosphatase; CGI 202; HSPC141; Phosphohistidine phosphatase 1; PHP14
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Species
Human
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Source
P. pastoris
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NRX4-1
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Expression Region
M1-Y125
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Protein Length
Full Length of Isoform-1
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PHPT1 (Phosphate-regulating gene with homologies to Endopeptidases on the X chromosome 1) is a putative protein implicated in the regulation of phosphate metabolism, particularly in the context of various physiological and pathological conditions. Research into PHPT1 has gained attention due to its potential role in conditions such as familial hypophosphatemic rickets, where mutations in phosphate-regulating genes lead to disrupted phosphate homeostasis. Understanding the function and regulation of PHPT1 could provide insights into the pathogenesis of phosphate-related disorders as well as other metabolic conditions involving mineral metabolism. The recombinant production of PHPT1 protein is critical for biochemical assays, enabling the exploration of its enzymatic function, structural characteristics, and interactions with other molecules. Such studies may facilitate the development of therapeutic interventions targeting dysregulated phosphate metabolism, thereby addressing a range of clinical challenges related to bone health and mineral deficiencies. Additionally, elucidating the role of PHPT1 in signaling pathways may reveal broader implications for its involvement in cellular processes beyond mineral regulation, thus providing a comprehensive understanding of its biological significance.
