Analytical Data
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Gene name
SAA1
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简介
SAA1 Protein, a major acute phase protein, forms a homohexamer comprising dimers of trimers. While the native form doesn't spontaneously form amyloid fibrils, partial proteolysis can induce amyloid fibril generation. SAA1 also serves as an apolipoprotein in the HDL complex and shows affinity for heparin. SAA1 Protein, Human (His) is the recombinant human-derived SAA1 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Biological Activity
Measured by its ability to chemoattract THP-1 cells. The ED50 for this effect is <100 ng/mL. Measured by its ability to chemoattract THP-1 cells. The ED50 for this effect is 53.85 ng/mL, corresponding to a specific activity is 1.857×10^4 U/mg.
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Alternative Names
Serum Amyloid A-1 Protein; SAA; SAA1
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
AAH07022.1
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Expression Region
R19-Y122
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Protein Length
Partial
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Molecular Weight
13.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SAA1 (serum amyloid A1) is an acute-phase protein primarily synthesized by the liver in response to inflammation, infection, and tissue injury. It plays a crucial role in the immune response and has been implicated in various inflammatory diseases, including atherosclerosis and rheumatoid arthritis. Research on SAA1 recombinant proteins has gained momentum as scientists seek to understand its structure, function, and potential therapeutic applications. The recombinant form of SAA1 allows for detailed studies of its biochemical properties, interactions with other proteins, and its role in amyloidogenesis, where misfolded proteins aggregate and form amyloid fibrils, contributing to diseases like systemic amyloidosis. Furthermore, as SAA1 levels can be indicative of various pathological conditions, its recombinant form is being explored as a biomarker for disease diagnosis and monitoring. Investigations into SAA1 also focus on its potential as a target for drug development, aiming to modulate its activity to mitigate inflammatory responses. Overall, the study of SAA1 recombinant proteins is crucial for elucidating the biological mechanisms underlying inflammation and amyloid-related disorders, paving the way for novel therapeutic strategies.
