Analytical Data
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基因名
Cytosolic beta-Glucosidase/GBA3
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简介
Cytosolic β-glucosidase/GBA3 is a multifunctional neutral cytosolic β-glucosidase that displays broad substrate specificity, suggesting a possible involvement in glycosylceramide catabolism. Although it exhibits significant glucosylceramidase activity in vitro, its in vivo relevance is unclear. Cytosolic beta-Glucosidase/GBA3 Protein, Human (GST) is the recombinant human-derived Cytosolic beta-Glucosidase/GBA3 protein, expressed by E. coli , with N-GST labeled tag.
- Application
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别名
CBG; CBGL1; Cytosolic beta glucosidase ; Cytosolic beta glucosidase like protein 1; Cytosolic beta-glucosidase; Glucosidase beta acid 3; Klotho related protein ; KLRP
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种属
Human
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表达系统
E. coli
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标签
N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H227-1
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表达区间
M1-L162
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cytosolic beta-Glucosidase (GBA3) is an important enzyme involved in the hydrolysis of beta-glucosidic bonds in various glycosides, and it plays a crucial role in carbohydrate metabolism and glycan degradation. Mutations in the GBA3 gene have been linked to several metabolic disorders, making it a target of interest in biomedical research. The enzyme's activity is particularly significant in the context of diseases such as Gaucher disease, where β-glucosylceramide accumulation occurs due to defective glucocerebrosidase activity, leading to secondary pathological changes. Understanding the structure and function of GBA3 is crucial for elucidating its role in both normal physiology and disease states. The recombinant production of GBA3 enables the study of its biochemical properties, substrate specificity, and interaction with inhibitors or other biomolecules. Moreover, characterizing the enzyme can aid in developing therapeutic strategies for conditions related to its dysfunction. Research involving GBA3, including enzyme kinetics, structural biology, and potential therapeutic applications, enhances our comprehension of glycosidase function and opens avenues for novel treatment options for glycosylation-related disorders.
