Analytical Data
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Gene name
Cytosolic beta-Glucosidase/GBA3
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简介
Cytosolic β-glucosidase/GBA3 is a multifunctional neutral cytosolic β-glucosidase that displays broad substrate specificity, suggesting a possible involvement in glycosylceramide catabolism. Although it exhibits significant glucosylceramidase activity in vitro, its in vivo relevance is unclear. Cytosolic beta-Glucosidase/GBA3 Protein, Human (GST) is the recombinant human-derived Cytosolic beta-Glucosidase/GBA3 protein, expressed by E. coli , with N-GST labeled tag.
- Application
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Alternative Names
CBG; CBGL1; Cytosolic beta glucosidase ; Cytosolic beta glucosidase like protein 1; Cytosolic beta-glucosidase; Glucosidase beta acid 3; Klotho related protein ; KLRP
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Species
Human
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Source
E. coli
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H227-1
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Expression Region
M1-L162
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cytosolic beta-Glucosidase (GBA3) is an important enzyme involved in the hydrolysis of beta-glucosidic bonds in various glycosides, and it plays a crucial role in carbohydrate metabolism and glycan degradation. Mutations in the GBA3 gene have been linked to several metabolic disorders, making it a target of interest in biomedical research. The enzyme's activity is particularly significant in the context of diseases such as Gaucher disease, where β-glucosylceramide accumulation occurs due to defective glucocerebrosidase activity, leading to secondary pathological changes. Understanding the structure and function of GBA3 is crucial for elucidating its role in both normal physiology and disease states. The recombinant production of GBA3 enables the study of its biochemical properties, substrate specificity, and interaction with inhibitors or other biomolecules. Moreover, characterizing the enzyme can aid in developing therapeutic strategies for conditions related to its dysfunction. Research involving GBA3, including enzyme kinetics, structural biology, and potential therapeutic applications, enhances our comprehension of glycosidase function and opens avenues for novel treatment options for glycosylation-related disorders.
