Analytical Data
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Gene name
CCNA2
- Application
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Alternative Names
(Cyclin-A)(Cyclin A)
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Species
Human
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P20248
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Expression Region
1-432aa
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Molecular Weight
54.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The DCAF16-DDB1-CUL4A-RBX1 complex plays a crucial role in the ubiquitin-proteasome system, a key pathway for protein degradation and regulation in cells. This multiprotein complex is part of the E3 ubiquitin ligase family, which facilitates the transfer of ubiquitin moieties to target proteins, marking them for degradation. DCAF16 serves as a substrate receptor, recognizing specific proteins for ubiquitination, while DDB1 acts as a scaffolding protein, linking DCAF16 to the CUL4A-RBX1 module, which is responsible for the actual ubiquitin transfer. Dysregulation of this complex has been implicated in various diseases, including cancer, where it may affect cell cycle regulation and apoptosis. Moreover, research into this complex provides insights into the mechanisms of protein homeostasis and the cellular response to stress. Understanding the structural and functional dynamics of the DCAF16-DDB1-CUL4A-RBX1 complex could lead to novel therapeutic strategies targeting the ubiquitin-proteasome system in pathological conditions. Recent studies have focused on elucidating the protein interactions within this complex and characterizing the specific substrates recognized by DCAF16, highlighting its importance in both fundamental biology and potential clinical applications.
