Analytical Data
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Gene name
BamA
- Application
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Alternative Names
Outer membrane protein assembly factor BamA(Omp85)
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Species
Escherichia coli
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Source
Yeast
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Tag
C- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A940
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Expression Region
175-424aa
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Molecular Weight
30 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BamA, an essential outer membrane protein in Gram-negative bacteria, plays a critical role in the assembly and stability of β-barrel proteins in the outer membrane. This protein, part of the β-barrel assembly machine (BAM) complex, is vital for maintaining the integrity of the bacterial envelope, facilitating the proper folding and insertion of various membrane proteins, which are crucial for cell viability and function. Given its significance in bacterial physiology and pathogenicity, BamA has emerged as a promising target for antibiotic development. Researchers have focused on engineering recombinant BamA proteins to study their structure, function, and interactions with other components of the BAM complex. These studies aim to elucidate the mechanisms underlying β-barrel protein assembly, providing insights into bacterial resistance and potential therapeutic strategies. By understanding BamA's role at a molecular level, scientists hope to identify novel approaches to disrupt its function, offering new avenues for combating antibiotic-resistant infections. The recombinant production of BamA not only facilitates basic research but also opens doors for high-throughput screening of small molecule inhibitors, contributing to the development of next-generation antibacterial agents.
