Analytical Data
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Gene name
pp1a
- Application
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Alternative Names
Replicase polyprotein 1a; ORF1a polyprotein; Avian infectious bronchitis virus (strain M41); IBV; pp1a; Hydrolase; Lyase; Protease; RNA-binding; Thiol protease
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Species
Virus
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Source
E. coli
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Tag
His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0C6V5
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Expression Region
G2783-Q3088
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PP1A, or protein phosphatase 1 alpha, is a critical serine/threonine phosphatase involved in various cellular processes, including cell division, apoptosis, and signaling pathways. It plays a key role in regulating the phosphorylation states of numerous substrates, thereby influencing critical physiological functions and cellular homeostasis. Research on PP1A has gained traction due to its implications in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, where dysregulation of phosphatase activity can lead to pathological conditions. Scientists have focused on characterizing PP1A's structure, function, and regulatory mechanisms to understand its role in health and disease better. Recombinant protein production of PP1A allows for detailed studies of its biochemical properties, interactions with other proteins, and post-translational modifications. Moreover, understanding PP1A's activity is crucial for developing targeted therapies that could modulate its function, offering potential avenues for innovative treatments. By employing modern techniques such as crystallography, mutagenesis, and high-throughput assays, researchers are unraveling the complexities of PP1A, paving the way for therapeutic interventions and enhancing our knowledge of phosphatase biology.
