Analytical Data
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Gene name
SKP1-SKP2
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简介
The SKP1 protein is a component of the SCF ubiquitin ligase complex and targets cell cycle progression and signal transduction. SKP1/SKP2 Protein, Human (Active) is the recombinant human-derived SKP1/SKP2, expressed by E. coli, with tag-free.
- Application
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Alternative Names
EMC19; OCP2; SKP1A; TCEB1L
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P63208 (M1-K163)&Q13309-1
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Expression Region
P63208 (M1-K163)&Q13309-1 (R89-L424)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SKP1 and SKP2 are crucial components of the SCF (SKP1-Cullin-F-box) E3 ubiquitin ligase complex, which plays a significant role in the regulation of protein degradation pathways through the ubiquitin-proteasome system. SKP1 serves as an adaptor protein that links F-box proteins, which recognize specific substrates, to the Cullin scaffold, thus facilitating the ubiquitination process. SKP2, an F-box protein, is known for its role in the cell cycle regulation by targeting the cyclin-dependent kinase inhibitor p27 for degradation, thereby promoting cell cycle progression and cellular proliferation. Dysregulation of SKP2 has been implicated in various cancers, making it a valuable target for therapeutic interventions. The study and characterization of SKP1-SKP2 recombinant proteins are essential for understanding the molecular mechanisms underlying their function in ubiquitination and cell cycle control. Researchers employ techniques like protein expression and purification to investigate the structural properties and interactions of these proteins, which can reveal potential drug targets or inform the development of inhibitors that disrupt abnormal protein degradation pathways in cancer. Overall, the exploration of SKP1-SKP2 recombination provides insights into the complex regulatory networks governing cellular processes and has significant implications for cancer therapy and other diseases related to protein homeostasis.
