Analytical Data
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Gene name
DCAF16
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简介
DCAF16 Protein, a vital component in the CUL4-DDB1 E3 ubiquitin-protein ligase complex, serves as a crucial substrate recognition element. It plays a central role in ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins, emphasizing its key involvement in protein modification processes, particularly in the regulation of protein ubiquitination. DCAF16/DDB1 Protein, Human (Active, Biotinylated, sf9) is the recombinant human-derived DCAF16/DDB1, expressed by Sf9 insect cells, with tag-free.
- Application
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Alternative Names
C4orf30
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Species
Human
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Source
Baculovirus
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NXF7 (G2-L216)&Q16531-1
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Expression Region
Q9NXF7 (G2-L216)&Q16531-1 (M1-H1140)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DCAF16, a member of the DCAFs (DDB1 and CUL4-associated factors) family, has garnered significant interest in the field of molecular biology and protein research due to its role in the ubiquitin-proteasome system (UPS). This system is crucial for regulating protein degradation and homeostasis within the cell, influencing various biological processes, including cell cycle control, DNA damage response, and immune responses. DCAF16 acts as a substrate receptor that interacts with DDB1 and CUL4, forming an E3 ubiquitin ligase complex that targets specific proteins for ubiquitination and subsequent degradation. Research into DCAF16 is vital, as dysregulation of ubiquitin-mediated degradation pathways is implicated in various diseases, including cancer, neurodegenerative disorders, and viral infections. Understanding the structure, function, and regulatory mechanisms of DCAF16 could illuminate new therapeutic targets and strategies. Recent studies have focused on the reconstitution of DCAF16 in vitro to investigate its substrate specificity and interaction partners, providing insights into its role in cellular processes. Additionally, exploring the effect of post-translational modifications on DCAF16 function and stability may enhance our understanding of its contribution to disease mechanisms. This research not only aims to delineate the biological significance of DCAF16 but also endeavors to leverage these findings for potential applications in drug discovery and the development of novel therapeutic approaches.
