Analytical Data
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基因名
ALK-1
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简介
ALK-1, also known as ACVRL1, is a type I receptor for TGF-β superfamily with 2 ligands, BMP9 and BMP10. ALK-1 is predominantly expressed in endothelial cells and plays a critical role in regulating developmental and pathological angiogenesis[1][2]. ACVRL1/ALK1 Protein, Mouse (HEK293, Fc) is produced in HEK293 cells with a C-Terminal Fc-tag. It consists of 97 amino acids (D23-P119).
- Application
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生物活性
1. This product does not contain protein kinase domain. 2. Measured by its ability to inhibit BMP-9-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 20-80 ng/mL in the presence of 2 ng/mL of rhBMP-9. Measured by its ability to inhibit BMP-9-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 60.50 ng/mL in the presence of 2 ng/mL of rhBMP-9, corresponding to a specific activity is 1.653×104 units/mg.
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别名
rMuActivin Receptor-like Kinase 1, C-Fc; ALK-1; ACVRL1; Activin Receptor-like Kinase 1
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种属
Mouse
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表达系统
HEK293
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标签
C-Fc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q61288
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表达区间
D23-P119
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蛋白长度
Extracellular Domain
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分子量
55-60 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ALK-1, or activin receptor-like kinase 1, is a type I receptor that plays a crucial role in various biological processes, including angiogenesis, endothelial cell function, and vascular homeostasis. Research has shown that ALK-1 is primarily involved in the transforming growth factor-beta (TGF-β) signaling pathway, influencing vascular development and repair. Dysregulation of ALK-1 has been implicated in several pathological conditions, including pulmonary arterial hypertension and hereditary hemorrhagic telangiectasia, highlighting its significance as a therapeutic target. The recombinant form of ALK-1 protein has garnered interest in scientific studies due to its potential applications in regenerative medicine and vascular biology. By generating ALK-1 recombinant protein, researchers aim to explore its biological functions, assess its interactions with other signaling molecules, and evaluate its potential as a biomarker or therapeutic agent. Such studies may facilitate a better understanding of vascular diseases and lead to novel treatment strategies.
