Analytical Data
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Gene name
Ketohexokinase/KHK
- Application
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Alternative Names
Ketohexokinase(EC 2.7.1.3)(Hepatic fructokinase)
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Species
Mouse
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Source
HEK293
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P97328
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Expression Region
1-298aa
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Molecular Weight
36.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Ketohexokinase (KHK), also known as fructokinase, is an important enzyme involved in the metabolic pathway of fructose and plays a crucial role in energy metabolism. It catalyzes the phosphorylation of fructose to fructose-1-phosphate, which is a pivotal step in fructose utilization. Abnormal KHK activity has been linked to various metabolic disorders, including obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Given the rising prevalence of these conditions, understanding the structure and function of KHK has garnered significant research interest. Recombinant KHK protein offers a valuable tool for elucidating its enzymatic properties and regulatory mechanisms, facilitating the development of novel therapeutic strategies targeting fructose metabolism. Additionally, functional studies utilizing recombinant KHK enable researchers to explore its role in metabolic pathways and the implications of its dysregulation in health and disease. As such, generating and characterizing recombinant KHK not only enhances our understanding of its biological function but also provides a foundation for exploring the potential of KHK as a target in metabolic disease therapies.
