Analytical Data
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基因名
Ketohexokinase/KHK
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简介
Ketohexokinase (KHK) proteins play a key role in carbohydrate metabolism by catalyzing the phosphorylation of ketoglycosides into fructose-1-phosphate. This enzyme activity is a key step in the utilization and processing of fructose within cells. Ketohexokinase/KHK Protein, Human (HEK293, His) is the recombinant human-derived Ketohexokinase/KHK protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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别名
rHuKetohexokinase/KHK, His; Ketohexokinase; Hepatic fructokinase; KHK
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
AAH06233.1
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表达区间
M1-V298
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氨基酸序列
MEEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSMAPGHVADFLVADFRRRGVDVSQVAWQSKGDTPSSCCIINNSNGNRTIVLHDTSLPDVSATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQLFGYGDVVFVSKDVAKHLGFQSAEEALRGLYGRVRKGAVLVCAWAEEGADALGPDGKLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSQGRSVQEALRFGCQVAGKKCGLQGFDGIV
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蛋白长度
Full Length
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分子量
30.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Ketohexokinase (KHK), also known as fructokinase, is an important enzyme involved in the metabolic pathway of fructose and plays a crucial role in energy metabolism. It catalyzes the phosphorylation of fructose to fructose-1-phosphate, which is a pivotal step in fructose utilization. Abnormal KHK activity has been linked to various metabolic disorders, including obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Given the rising prevalence of these conditions, understanding the structure and function of KHK has garnered significant research interest. Recombinant KHK protein offers a valuable tool for elucidating its enzymatic properties and regulatory mechanisms, facilitating the development of novel therapeutic strategies targeting fructose metabolism. Additionally, functional studies utilizing recombinant KHK enable researchers to explore its role in metabolic pathways and the implications of its dysregulation in health and disease. As such, generating and characterizing recombinant KHK not only enhances our understanding of its biological function but also provides a foundation for exploring the potential of KHK as a target in metabolic disease therapies.
