Analytical Data
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Gene name
CD39L4/ENTPD5
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简介
The CD39L4/ENTPD5 protein is located in the lumen of the endoplasmic reticulum and plays a crucial role in glycoprotein processing by hydrolyzing nucleoside diphosphates (including GDP, IDP, and UDP). Its enzymatic activity is critical for the clearance of UDP, an end-product feedback inhibitor of UDP-Glc:glycoprotein glucosyltransferase. CD39L4/ENTPD5 Protein, Mouse (sf9, His) is the recombinant mouse-derived CD39L4/ENTPD5 protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Application
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Alternative Names
Ectonucleoside triphosphate diphosphohydrolase 5; NTPDase 5; CD39 antigen-like 4; CD39L4
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Species
Mouse
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Source
Baculovirus
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9WUZ9
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Expression Region
T19-S427
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Protein Length
Partial
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Molecular Weight
45 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD39L4, also known as ENTPD5, is a member of the nucleoside triphosphate diphosphohydrolase (NTPDase) family, which plays a crucial role in regulating extracellular nucleotide levels. This protein is involved in the hydrolysis of ATP and ADP to AMP, thereby influencing purinergic signaling pathways that are essential for various physiological processes, including immune response and cell proliferation. Recent research has highlighted the significance of CD39L4 in cancer biology, as it may facilitate tumor immune evasion by modulating the local concentration of extracellular ATP, a key signal in immune cell activation. Investigating the recombinant expression of CD39L4/ENTPD5 is crucial for understanding its biochemical properties and potential therapeutic applications. By producing this recombinant protein, researchers aim to elucidate its structure-function relationship and explore its role in the tumor microenvironment. Additionally, understanding the mechanisms through which CD39L4 influences purinergic signaling could open new avenues for targeted cancer therapies and immune modulation. As such, CD39L4/ENTPD5 is a promising target for further investigation, with implications for developing strategies that enhance anti-tumor immunity and improve patient outcomes in various cancers.
