Analytical Data
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Gene name
RAB27B
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简介
RAB27B is a dynamic small GTPase that regulates the late endocytic pathway by cycling between its active GTP-bound and inactive GDP-bound states. In the active state, RAB27B interacts with multiple effectors to coordinate endosomal localization, maturation, and secretion. RAB27B Protein, Human (HEK293, His) is the recombinant human-derived RAB27B protein, expressed by HEK293 , with N-His labeled tag.
- Application
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Alternative Names
Ras-related protein Rab-27B; C25KG; RAB27B
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Species
Human
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Source
HEK293
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00194
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Expression Region
T2-K215
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Protein Length
Full Length of Mature Protein
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Molecular Weight
29 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RAB27B is a member of the RAB GTPase family, which plays a crucial role in intracellular vesicle trafficking and exocytosis. It is particularly involved in the regulation of secretory pathways in various cell types, including melanocytes, platelets, and immune cells. The study of RAB27B has gained prominence due to its significant implications in various physiological and pathological processes, including pigmentation, immune response, and cancer metastasis. Dysregulation of RAB27B has been linked to several diseases, such as Griscelli syndrome, which is characterized by immunological defects and hypopigmentation. Furthermore, RAB27B is involved in the secretion of cytotoxic granules in T cells and natural killer (NK) cells, making it a potential target for immunotherapy. The recombinant expression of RAB27B in different host systems allows researchers to investigate its functional roles, explore its interaction with effector proteins, and study its involvement in cellular processes. Understanding the molecular mechanisms of RAB27B can provide insights into its contributions to disease, paving the way for novel therapeutic strategies that could modulate its activity for clinical benefits. As such, RAB27B has become a focal point in cell biology and therapeutic research aimed at addressing conditions linked to its dysfunction.
