Analytical Data
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Gene name
LCAT
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简介
LCAT protein is synthesized in the liver and plays a vital role in the metabolism of plasma lipoproteins by converting cholesterol and phosphatidylcholine in HDL and LDL. LCAT Protein, Human (HEK293, His) is the recombinant human-derived LCAT protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P04180
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Expression Region
F25-E440
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AA Sequence
FWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE
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Protein Length
Full Length of Mature Protein
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Molecular Weight
71 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LCAT, or lecithin-cholesterol acyltransferase, is a crucial enzyme in lipid metabolism, primarily responsible for the conversion of free cholesterol into cholesteryl esters, which are subsequently transported in lipoproteins. The significance of LCAT in cardiovascular health is highlighted by its role in the reverse cholesterol transport pathway, where it aids in the removal of excess cholesterol from peripheral tissues and its delivery to the liver for excretion. Dysregulation of LCAT activity has been associated with various lipid disorders, including high-density lipoprotein (HDL) dysfunction and atherosclerosis, making it a target for therapeutic interventions. Research into recombinant LCAT (rLCAT) has gained momentum as scientists aim to better understand its structure-function relationships and to develop potential biotherapeutics for dyslipidemia and related cardiovascular diseases. By employing recombinant DNA technology, researchers can produce LCAT in vitro, allowing for detailed biochemical analyses and the exploration of its enzymatic mechanisms. This has led to increased interest in potential strategies to enhance LCAT activity or to develop LCAT mimetics, which could help in mitigating the effects of cardiovascular diseases linked to lipid imbalances. As the prevalence of cardiovascular diseases rises worldwide, LCAT represents a promising avenue for innovative treatments, emphasizing the importance of ongoing research in this domain.
