Analytical Data
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基因名
DNA polymerase beta
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简介
DNA polymerase beta Protein, Human (His), a DNA repair enzyme, is involved in various types of DNA repair. DNA polymerase beta Protein performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance.
- Application
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别名
rHuDNA polymerase beta, His; DNA Polymerase Beta; POLB
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种属
Human
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表达系统
E. coli
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P06746
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表达区间
S2-E335
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氨基酸序列
SKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE
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蛋白长度
Partial
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分子量
39.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNA polymerase beta (pol β) is a crucial enzyme involved in base excision repair, a cellular mechanism that corrects DNA damage resulting from oxidative stress, environmental factors, or normal metabolic processes. This enzyme plays a pivotal role in maintaining genomic stability, as it fills in gaps generated during the repair of single-strand breaks and is involved in the synthesis of DNA in various physiological processes. Research on pol β has garnered significant interest because its dysregulation is implicated in various diseases, including cancer, neurodegenerative disorders, and aging. Studies have revealed that pol β possesses both polymerase and exonuclease activities, allowing it to accurately insert nucleotides and proofread newly synthesized DNA. Understanding the structure-function relationship of pol β, as well as how it interacts with other proteins in the DNA repair pathway, is vital for developing therapeutic strategies aimed at enhancing DNA repair mechanisms or targeting dysregulated pol β in tumorigenesis. Recombination techniques have been employed to produce recombinant forms of pol β for detailed biochemical and structural studies, paving the way for novel insights into its catalytic mechanisms and potential as a drug target. Exploring pol β's role in DNA repair and its implications in disease progression highlights the importance of this enzyme in molecular biology and medicine.
