Analytical Data
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Gene name
DNA polymerase beta
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简介
DNA polymerase beta Protein, Human (His), a DNA repair enzyme, is involved in various types of DNA repair. DNA polymerase beta Protein performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance.
- Application
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Alternative Names
rHuDNA polymerase beta, His; DNA Polymerase Beta; POLB
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Species
Human
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Source
E. coli
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06746
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Expression Region
S2-E335
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AA Sequence
SKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE
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Protein Length
Partial
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Molecular Weight
39.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNA polymerase beta (pol β) is a crucial enzyme involved in base excision repair, a cellular mechanism that corrects DNA damage resulting from oxidative stress, environmental factors, or normal metabolic processes. This enzyme plays a pivotal role in maintaining genomic stability, as it fills in gaps generated during the repair of single-strand breaks and is involved in the synthesis of DNA in various physiological processes. Research on pol β has garnered significant interest because its dysregulation is implicated in various diseases, including cancer, neurodegenerative disorders, and aging. Studies have revealed that pol β possesses both polymerase and exonuclease activities, allowing it to accurately insert nucleotides and proofread newly synthesized DNA. Understanding the structure-function relationship of pol β, as well as how it interacts with other proteins in the DNA repair pathway, is vital for developing therapeutic strategies aimed at enhancing DNA repair mechanisms or targeting dysregulated pol β in tumorigenesis. Recombination techniques have been employed to produce recombinant forms of pol β for detailed biochemical and structural studies, paving the way for novel insights into its catalytic mechanisms and potential as a drug target. Exploring pol β's role in DNA repair and its implications in disease progression highlights the importance of this enzyme in molecular biology and medicine.
