Analytical Data
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Gene name
PDK1
- Application
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Alternative Names
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q15118
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Expression Region
Tyr233~Met430
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Molecular Weight
27kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PDK1 (3-phosphoinositide-dependent protein kinase-1) is a crucial regulator of several signaling pathways, particularly those involved in cell growth, survival, and metabolism. Its pivotal role in phosphorylating and activating members of the AKT family of protein kinases makes it a key player in the PI3K/AKT signaling cascade, which is often dysregulated in various cancers. Given its involvement in oncogenic processes, PDK1 has garnered significant interest as a potential therapeutic target for cancer treatment. Researchers are increasingly focusing on the production and characterization of recombinant PDK1 proteins to elucidate their biochemical properties and interactions. By utilizing techniques such as recombinant DNA technology, scientists can generate large quantities of PDK1, enabling detailed studies on its enzymatic activity, structural characteristics, and regulatory mechanisms. Additionally, understanding the differences between wild-type and mutant forms of PDK1 can provide insights into its function in pathological conditions. Overall, the investigation of recombinant PDK1 not only enhances our understanding of fundamental biological processes but also opens new avenues for drug development aimed at targeting PDK1-related pathways in cancer and other diseases.
