Analytical Data
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基因名
PDK1
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简介
PDK1 protein is a key kinase that controls glucose and fatty acid metabolism by phosphorylating pyruvate dehydrogenase subunits PDHA1 and PDHA2. This molecular switch inhibits pyruvate dehydrogenase, fine-tunes metabolite flux and downregulates aerobic respiration. PDK1 Protein, Human (sf9, His) is the recombinant human-derived PDK1 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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别名
Pyruvate dehydrogenase kinase isoform 1; PDK1; PDHK1
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种属
Human
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表达系统
Baculovirus
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15118
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表达区间
S29-A436
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蛋白长度
Full Length of Mature Protein
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分子量
45 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PDK1 (3-phosphoinositide-dependent protein kinase-1) is a crucial regulator of several signaling pathways, particularly those involved in cell growth, survival, and metabolism. Its pivotal role in phosphorylating and activating members of the AKT family of protein kinases makes it a key player in the PI3K/AKT signaling cascade, which is often dysregulated in various cancers. Given its involvement in oncogenic processes, PDK1 has garnered significant interest as a potential therapeutic target for cancer treatment. Researchers are increasingly focusing on the production and characterization of recombinant PDK1 proteins to elucidate their biochemical properties and interactions. By utilizing techniques such as recombinant DNA technology, scientists can generate large quantities of PDK1, enabling detailed studies on its enzymatic activity, structural characteristics, and regulatory mechanisms. Additionally, understanding the differences between wild-type and mutant forms of PDK1 can provide insights into its function in pathological conditions. Overall, the investigation of recombinant PDK1 not only enhances our understanding of fundamental biological processes but also opens new avenues for drug development aimed at targeting PDK1-related pathways in cancer and other diseases.
