Analytical Data
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Gene name
PGK1
- Application
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Species
Cricetulus griseus
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Source
Yeast
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Tag
C- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P50310
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Expression Region
2-417aa
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Molecular Weight
45.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PGK1, or phosphoglycerate kinase 1, is a crucial glycolytic enzyme that plays a significant role in cellular metabolism by catalyzing the conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, while facilitating the transfer of a phosphate group to ADP, resulting in ATP generation. The importance of PGK1 extends beyond its metabolic function, as its expression and activity have been linked to various physiological and pathological conditions, including cancer, where altered glycolytic pathways contribute to tumor growth and survival. Research into recombinant PGK1 has gained prominence due to its potential applications in biotechnology and medicine. Producing PGK1 as a recombinant protein allows for in-depth structural and functional studies, aiding in the understanding of its catalytic mechanism and regulation. Furthermore, PGK1 is being investigated as a potential biomarker for certain diseases and a target for therapeutic interventions. The protein's involvement in metabolic disorders also highlights its significance in developing strategies for disease management. Advances in recombinant DNA technology enable efficient production and purification of PGK1, facilitating high-throughput assays and drug screening. Overall, PGK1 research not only enriches our knowledge of fundamental metabolic processes but also opens avenues for innovative therapeutic approaches, making it a compelling subject for ongoing scientific investigation.
