Analytical Data
-
基因名
PGK1
-
简介
The PGK1 protein plays a key role in the glycolysis pathway by catalyzing one of two ATP-generating reactions, promoting the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate. In addition to its glycolytic function, PGK1 appears to act as a cofactor protein for polymerase α, playing a role in primer recognition. PGK1 Protein, Human (HEK293, His) is the recombinant human-derived PGK1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
-
别名
Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2; PGK1; PGKA
-
种属
Human
-
表达系统
HEK293
-
标签
C-6*His
-
纯度
Greater than 95% as determined by SDS-PAGE.
-
蛋白编号
P00558-1
-
表达区间
S2-I417
-
氨基酸序列
SLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI
-
蛋白长度
Full Length of Isoform-1 Mature Protein
-
分子量
46-50 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PGK1, or phosphoglycerate kinase 1, is a crucial glycolytic enzyme that plays a significant role in cellular metabolism by catalyzing the conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, while facilitating the transfer of a phosphate group to ADP, resulting in ATP generation. The importance of PGK1 extends beyond its metabolic function, as its expression and activity have been linked to various physiological and pathological conditions, including cancer, where altered glycolytic pathways contribute to tumor growth and survival. Research into recombinant PGK1 has gained prominence due to its potential applications in biotechnology and medicine. Producing PGK1 as a recombinant protein allows for in-depth structural and functional studies, aiding in the understanding of its catalytic mechanism and regulation. Furthermore, PGK1 is being investigated as a potential biomarker for certain diseases and a target for therapeutic interventions. The protein's involvement in metabolic disorders also highlights its significance in developing strategies for disease management. Advances in recombinant DNA technology enable efficient production and purification of PGK1, facilitating high-throughput assays and drug screening. Overall, PGK1 research not only enriches our knowledge of fundamental metabolic processes but also opens avenues for innovative therapeutic approaches, making it a compelling subject for ongoing scientific investigation.
