Analytical Data
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Gene name
NONO
- Application
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Alternative Names
P54; NMT55; NRB54; P54NRB; Nuclear RNA-Binding Protein 54-kD; 54 kDa nuclear RNA- and DNA-binding protein; DNA-binding p52/p100 complex, 52 kDa subunit
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Species
Human
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Source
E. coli
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Tag
N- His & GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15233
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Expression Region
Lys18~Asp228
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Molecular Weight
54kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NONO, a member of the non-POU domain containing octamer-binding protein family, plays a crucial role in various cellular processes, including RNA metabolism, transcription regulation, and stress response. Its involvement in the regulation of gene expression and splicing mechanisms has garnered significant attention in the field of molecular biology. Recent studies have highlighted NONO's dual functions in both nuclear processes and the cytoplasmic response to stress, indicating its importance in maintaining cellular homeostasis. Additionally, NONO has been implicated in several diseases, including cancer and neurodegenerative disorders, where its dysregulation can contribute to pathogenesis. Research focusing on the structure and dynamics of NONO, particularly its protein-protein interactions and post-translational modifications, is vital for understanding its diverse functions. Emerging evidence suggests that NONO may serve as a potential therapeutic target, making it imperative to deepen our understanding of its biochemical properties and the impact of its restructured forms in various biological contexts.
